Introduction to Microscopy Exam 04.03.2020

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Mryoyo15
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Introduction to Microscopy Exam 04.03.2020

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  1. Draw a schematic setup of SEM including detectors
  2. Describe the different method of cryo fixation of biological samples
  3. What steps of preparation are necessary for cryo fixation freeze substitution
  4. Why does fluorescent light have a longer wavelength? How is this phenomenon called? What is the fluorescent lifetime?
  5. What properties do fluorescent molecules possess and what defines their color?
  6. Describe the principle of polarization microscopy
  7. Put the steps for Köhler Illumination into order
  8. What is the critical illumination. Discuss its advantages and disadvantages
  9. Label the microscope
  10. What is the numerical aperture? How can it be calculated and what defines it?

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