Exam 20.12.2021

[aero_penguin]

Answer 10 out of 11 questions in 60 minutes.

1. SEM vs. ESEM. Discuss advantages and disadvantages. Make a sketch.
2. Explain cryotomography. Make a sketch.
3. Explain high pressure freezing.
4. What is a fluorophore? What properties does it have?
5. Advantages of flourescence microscopy compared to other techniques?
6. How can you label structures with a fluorophore?
7. Explain and sketch spherical abberation and comatic abberation.
8. What are the resolution limits for the eye/light microscope/electron microscope? Give examples for structures that you can view with each.
9. Advantages and disadvantages when using a upright microscope on a biological sample.
10. Bring Köhler illumination steps in the right order.
11. Label the microscope.